Below are some of the results obtained with the logical cis-regulatory control model for the Endo16 gene. Observed expression data using various CAT constructs are plotted as filled circles, and the data calculated on the basis of the model on the previous page are represented by the open circles. The full construct (control) is indicated by the black data, the data for constructs in which one or more locus was mutated are in different colours. See the references on the previous page for further details.
| Data (obs = observed experimentally; calc = calculated from the model, using the input data below) | ||||||||||||
| Construct |
1 |
2 | 3 | 4 | 5 | 6 | ||||||
| Time (hrs) | obs | calc | obs | calc | obs | calc | obs | calc | obs | calc | obs | calc |
| 30 | 0.28 | 0.32 | 0.23 | 0.32 | 0.16 | 0.19 | 0.29 | 0.32 | 0.44 | 0.32 | 0.2 | 0.32 |
| 48 | 0.62 | 0.52 | 0.45 | 0.51 | 0.32 | 0.25 | 0.51 | 0.4 | 0.97 | 1.02 | 0.34 | 0.51 |
| 72 | 1 | 1 | 0.29 | 0.19 | 0.12 | 0.19 | 0.88 | 0.76 | 1.03 | 1.18 | 0.28 | 0.45 |
|
CAT constructs |
|
|
1 |
BA-Bp.CAT |
|
2 |
B(Uim)A-Bp.CAT |
|
3 |
B(CB2m)A-Bp.CAT |
|
4 |
B(Cym,CB1m)A-Bp.CAT |
|
5 |
B(Rm)A-Bp.CAT |
|
Input values |
||||||
|
Time (hrs) |
UI |
R |
k |
CB2 (driver) |
CB2 (amp) |
OTX |
|
30h |
0 |
0 |
1 |
0.5 |
0 |
0.75 |
|
48h |
1 |
1 |
2 |
1 |
1 |
1 |
|
72h |
1 |
1 |
2 |
1 |
1 |
0.75 |